2016年はち月にじゅう日、農(nóng)業(yè)部農(nóng)業(yè)ゲノムサイエンス學(xué)科群が始めた「重要穀物參考ゲノム計(jì)畫セミナー」で、専門家は「単一のゲノムはもうない代表個(gè)體を自身のゲノムの特徴で、同じ種の違う品係、違う群體間の違いは個(gè)體に影響が現(xiàn)れた形質(zhì)?！工长欷?、遺伝資源情報(bào)を十分に発掘し、種の進(jìn)化や作物の適応メカニズムを全面的に探っていきたいと指摘した場(chǎng)合には、種の遺伝子を構(gòu)築することが必要です。

ソリューション：10X Genomicsゲノムシークエンシング

に対して分子育種分野の発展趨勢(shì)、博奧晶典linked-readsますコーディネート二代目シークエンシングのシークエンシングサービスが生まれて。（前にも10X Genomicsプラットフォームの詳細(xì)を紹介し、小さく編む贅言ない、文末に直行リンクをクリックしてください。

二代に比べて三代目とシークエンシング、10X Genomicsゲノムシークエンシング質(zhì)の高い、組み立ての効果の良い、期間が短い。三代に比べて1ゲノムのコストを使用し、10X Genomics測(cè)以上のゲノムデータ、価格より高い。10X Genomicsプラットフォームの2つの応用方向の特徴は次の通り：

10X Genomicsのnovoシークエンシング
?大スパンlinked-readsを正確にContig組み立てScaffoldの基礎(chǔ)を築く、
?だけで構(gòu)築10X Genomics文庫、超低コスト、
?組み立てアルゴリズムのzui適化は、組み立てサイクルを大幅に短縮した、
すでに?にゲノムの序列を延長(zhǎng)Scaffold有効長(zhǎng)さを獲得し、より質(zhì)の高いゲノム情報(bào)。
10X Genomics動(dòng)植物重シークエンシング
シークエンシング?コスト削減とともに、SVの研究より多くの注目を受け、
?SV検出の正確率向上に30 %以上、
?技術(shù)が斬新で、結(jié)果は信頼され、結(jié)果は可視化し、文章を発表するのは比較的容易である。

博奧晶典プロジェクト経験が豊富で、すでに成功して300 +の提供することができます、安定性と信頼性の高い結(jié)果。

ケース表示1

前バージョンに唐辛子を発表している編（NG、PNAS）は、3つのバージョンでは、そのzui大のContig N50は55k、zui大のScaffold N50は2.47M；今年はbioRxiv発表の新バージョンを利用し10X Genomics組み立て、Contigは前の2倍以上、そのContig N50を123kb、Scaffold N50を3.69M；ゲノム研究を構(gòu)築後辛味関連遺伝子発見pun1、辛くないの唐辛子の中でpun1遺伝子が2.57kの欠如。これは他のバージョンの組み立てで発見されていません。

Have you ever wondered whether the partners of molecular breeding have had such doubts: the sample size has been very large, the group design is perfect, and a lot of sequencing work has been done, but still no character related gene is found? The reference genome is not good for use? The varieties of reference genomes and studies are too different?

Most of the reasons are: the genes related to this trait do not exist in the reference genome; many of the trait related genes are in the hypervariable region, which vary greatly, and cannot be identified by traditional methods.

In August 20, 2016, by the Ministry of agriculture genomics discipline group launched the "important crop reference genome project seminar, experts pointed out:" the genomic characteristics of single genome has been unable to represent different individual differences, the same species of different strains, different groups tend to affect the inter individual traits." This reminds us that if we want to fully explore the information of genetic resources, explore species evolution and crop adaptation mechanism comprehensively, we need to assemble and construct pan genomes or multiple genome information of species.

Solution: 10X Genomics genome sequencing

In the field of molecular breeding such trends, Boao crystal code linked-reads database collocation two generation sequencing sequencing services came into being. (before the detailed introduction of the 10X Genomics platform, the small editor will not say, please click the end of the link to direct.)

Compared with the two and three generation sequencing, the 10X Genomics genome sequencing was of high quality, good assembly effect and short cycle. Compared with the cost of making a single genome for the three generation, multiple genomic data can be measured with 10X Genomics, with a higher cost performance. The characteristics of the two application directions of the 10X Genomics platform are as follows:

10X Genomics De novo sequencing
The large span linked-reads lays the foundation for the accurate assembly of Contig into Scaffold.
It is necessary to build only a 10X Genomics library, which is very low in cost;
The optimization of assembly algorithm greatly shortens the assembly cycle.
For the existing genome sequences, the length of Scaffold can be extended effectively to obtain higher quality genomic information.
10X Genomics animal and plant re sequencing
With the reduction of sequencing cost, more and more attention has been paid to the research of SV.
The accuracy of SV detection was increased by more than 30%.
The technology is novel, the result is reliable, the result is visualized, and the publication of the article is relatively easy.

Boao crystal code project experience, has been building 300+, can provide stable and reliable results.

Case show one

Before the pepper version has been published 2 articles (NG, PNAS), there are three versions, one of the biggest Contig N50 is 55k, the largest Scaffold N50 2.47M; new version published this year in bioRxiv using the 10X Genomics assembly, Contig is two times more than before, the Contig N50 123KB, Scaffold N50 3.69M; Study on the construction of the genome was found after heat related gene pun1, found that deletion of pun1 not spicy pepper 2.57k gene. This is not found in the other versions of the assembly.

上一篇：11q23及DLEU1基因探針

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