	沙門氏菌核酸檢測試劑盒（PCR-熒光探針法）
	志賀氏菌（痢疾桿菌）核酸檢測試劑盒（PCR-熒光探針法）
	沙門氏菌/志賀氏菌雙通道核酸檢測試劑盒（PCR-熒光探針法）
	傷寒桿菌核酸檢測試劑盒（PCR-熒光探針法）
	副傷寒桿菌核酸檢測試劑盒（PCR-熒光探針法）
	甲型/乙型/丙型副傷寒桿菌核酸檢測試劑盒（PCR-熒光探針法）
	副溶血弧菌核酸檢測試劑盒（PCR-熒光探針法）
	大腸桿菌O157核酸檢測試劑盒（PCR-熒光探針法）
	腸出血性大腸桿菌O104:H4(aggR、flic、wzy)核酸檢測試劑盒（PCR-熒光探針法）
	腸出血性大腸桿菌志賀毒素（stx1/stx2）核酸檢測試劑盒（PCR-熒光探針法）
	腸產(chǎn)毒性大腸桿菌核酸檢測試劑盒（PCR-熒光探針法）
	腸粘附性大腸桿菌核酸檢測試劑盒（PCR-熒光探針法）
	腸侵襲性大腸桿菌（EIEC）核酸檢測試劑盒（PCR－熒光探針法）
	腸出血性大腸桿菌核酸檢測試劑盒（PCR－熒光探針法）
	腸致病性大腸桿菌核酸檢測試劑盒（PCR－熒光探針法）
	霍亂弧菌通用型核酸檢測試劑盒（PCR-熒光探針法）
	霍亂弧菌O1型核酸檢測試劑盒（PCR-熒光探針法）
	霍亂弧菌O139型核酸檢測試劑盒（PCR-熒光探針法）
	霍亂弧菌CTX基因核酸檢測試劑盒（PCR-熒光探針法）
	霍亂弧菌通用型、O1型、O139型、CTX基因四通道核酸檢測試劑盒（PCR-熒光探針法）
	小腸耶爾森菌核酸檢測試劑盒（PCR-熒光探針法）
	單核增生性李斯特氏菌核酸檢測試劑盒（PCR-熒光探針法）
	金黃色葡萄球菌核酸檢測試劑盒（PCR-熒光探針法）
	綠膿桿菌核酸檢測試劑盒（PCR-熒光探針法）
	空腸彎曲菌核酸檢測試劑盒（PCR-熒光探針法）
	擬態(tài)弧菌核酸檢測試劑盒（PCR-熒光探針法）
	河弧菌核酸檢測試劑盒（PCR-熒光探針法）
	創(chuàng)傷弧菌核酸檢測試劑盒（PCR-熒光探針法）
	溶藻弧菌核酸檢測試劑盒（PCR-熒光探針法）
	蠟樣芽胞桿菌核酸檢測試劑盒（PCR-熒光探針法）
	阪崎腸桿菌核酸檢測試劑盒（PCR-熒光探針法）
	產(chǎn)氣莢膜梭菌核酸檢測試劑盒（PCR-熒光探針法）
	A組輪狀病毒核酸檢測試劑盒（PCR-熒光探針法）
	B組輪狀病毒核酸檢測試劑盒（PCR-熒光探針法）
	C組輪狀病毒核酸檢測試劑盒（PCR-熒光探針法）
	諾沃克病毒核酸檢測試劑盒（PCR-熒光探針法）
	諾沃克病毒GI型核酸檢測試劑盒（PCR-熒光探針法）
	諾沃克病毒GⅡ型核酸檢測試劑盒（PCR-熒光探針法）
	札如病毒核酸檢測試劑盒（PCR-熒光探針法）
	星狀病毒核酸檢測試劑盒（PCR-熒光探針法）
	腸道腺病毒核酸檢測試劑盒（PCR-熒光探針法）
	諾沃克病毒GI/GⅡ雙通道核酸檢測試劑盒（PCR-熒光探針法）
	諾沃克病毒GI/GⅡ/A組輪狀病毒三通道核酸檢測試劑盒（PCR-熒光探針法）
	A組輪狀病毒/諾沃克病毒雙通道核酸檢測試劑盒（PCR-熒光探針法）

The principle and scope of application of Escherichia coli 0157 test tablets

1, principle and scope of application

Escherichia coli 0157 test piece is a pre prepared, disposable medium product, containing selective media and specific enzyme chromogenic agent, and colony on the test piece is blue, which can shorten counting time and enhance counting effect. This product is suitable for the determination of Escherichia coli 0157:H7 in all kinds of food and drinking water.

2. Methods of use

2.1, sample treatment: take sample 25mL (g) into the sampling tank or homogenized cup containing 225mL sterilized phosphate buffer diluent (or normal saline), make the 1:10 sample homogenate, and adjust the sample homogenization pH to 6.6 to 7.2 when necessary, with 1mol/LNaOH or 1mol/L HCl solution. Sterilize the pipette with 1mL, absorb the 1:10 sample and homogenize 1mL, pour into the test tube containing 9mL diluent, shake it up and become the sample of 1:100, homogenize it, and so on, and make the sample such as 1:1000 dilution to homogenize, and change one suction tube at a time.

2.2, inoculation: general food should be selected by 2~3 dilution tests. Liquid samples containing less bacteria (such as drinking pure water and mineral water) can be directly extracted from the original liquid for testing. The test piece was placed on the flat test table, the upper membrane was uncovered, the 1mL sample was collected by aseptic pipette, and the sample was uniformly distributed to the paper. The top layer was slowly covered and placed at 10s for about two pieces. At the same time, a blank negative control was made.

2.3, c*tion: put the test pieces together and put them back in the original self sealed bag and seal them. The transparent face is placed in the incubator with constant temperature, and the number of stacked pieces is not more than 12 pieces. The temperature of general food culture is 36? 1 C and 15 ~ 24h, and the culture temperature of aquatic products is 30 and 1 C, and the culture is 48h.

3, result interpretation

After culture, the purple red colonies on the test tablets were Escherichia coli 0157:H7, and the blue colony was the colony of other coliform colony. Samples of positive colonies are best verified by other more reliable methods, and at least repeat samples of the samples without conditions.

上一篇：諾如病毒G2型核酸PCR檢測試劑盒

下一篇：沙門氏/志賀氏菌雙通道核酸檢測試劑盒

返回列表>>

廣州健侖生物科技有限公司(www.newloor.com) 熱門產(chǎn)品:喹諾酮類檢測試劑盒,西尼羅河檢測試劑,基孔肯雅熱試劑,寨卡檢測試劑,疫病核酸試劑
地址：廣東省廣州市番禺區(qū)石樓鎮(zhèn)清華科技園創(chuàng)啟路63號A2棟101 Email：712628584@qq.com
ICP備：粵ICP備11063766號 GoogleSitemap 技術(shù)支持：化工儀器網(wǎng) 管理登陸返回首頁